The protective enzyme (SOD, POD, CAT) activities and soluble protein content of pistil after pollination were compared between interspecific-cross and self-cross of cultivar, using Tulipa specie (T. tianschanica) from Xinjiang and T. gesneriana cultivars ('Kees Nelis’, 'Negrita’) as test materials. The results showed that all the SOD, POD, CAT activities and soluble protein content changed after interspecific and self pollination, which means that the recognition between pollen and stigma can be initiated effectively when pollen fell on stigma despite of genetic relationship. Fluctuant changes were observed in 5 hours after pollination for protective enzyme activities and soluble protein content, and the fluctuation tendencies were different between interspecific and self pollination, as well as between 'Kees Nelis’ ×T. tianschanica and 'Negrita’×T. tianschanica. Self pollination appeared peaks of POD and CAT activities preceding interspecific pollination. Self pollination showed a larger rise and maintained rise for longer time than interspecific pollination. The SOD activity of 'Kees Nelis’ ×T. tianschanica changed more than that of 'Negrita’×T. tianschanica, meanwhile, the soluble protein content of the former declined preceding that of the latter. These differences were probably attributed to genetic relationship.

通过基本培养基(改良MS、改良KC、V&W及自制ZW系列)、植物激素(6-BA、KT、NAA、IBA)、培养条件(香蕉泥、蛋白胨、酵母提取物、糖、活性炭)等关键因子对文心兰组培各阶段影响的试验,探索了文心兰规模化组培育苗的关键技术。结果表明:(1)当6-BA 0.1～2 mg.L-1,NAA 0.1～1.0 mg.L-1时,可诱导拟原球茎形成与增殖;6-BA0.05～0.1 mg.L-1或附加NAA 0.1 mg.L-1就可满足拟原球茎的分化;6-BA 0.01 mg.L-1协同NAA或IBA 0.1mg.L-1处理,诱导生根快而粗壮;(2)添加活性炭1 000 mg.L-1和维生素C 30 mg.L-1对减轻外植体褐化是必要的;添加500～1 000 mg.L-1蛋白胨或酵母提取物可促进诱导拟原球茎;香蕉泥100 g.L-1有助于拟原球茎分化和瓶苗健化;(3)MS高盐类培养基在每个培养阶段都有较高的致畸率,自制中盐ZW培养基,N∶P∶K为3∶1∶2.9,文心兰在其中生长健壮;(4)文心兰在无糖培养基中自养生长基本正常。